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( E) Day 14 adipogenic colony with uniformly GFP+ stromal cells and PLIN1+ adipocytes. Nearby RFP+ fibroblasts show early signs of conversion (GFP+ perinuclear granules, white arrowheads). ( D) Day 14 adipogenic colony demonstrating PLIN1+ lipid droplets (pink, white arrows) in GFP+ adipocytes.

( C) Representative mixed colony with both GFP+ and RFP+ fibroblasts demonstrating GFP+ perinuclear granules in red cells (white arrowheads), indicating upregulation of adiponectin expression.

( B) Quantification of GFP and RFP expression in fibroblastic stromal cells within each culture dish (n = 3 independent mice, 166 total colonies counted). In addition, spontaneous adipogenesis was assessed based on immunostaining for perilipin 1 (PLIN1, pink). Endogenous fluorescence was then amplified by immunostaining for green fluorescent protein (GFP) and red fluorescent protein (RFP). IT IS A VIOLATION PUNISHABLE UNDER LAW FOR ANY PERSON UNDER THE AGE OF TWENTY-ONE TO PRESENT ANY WRITTEN EVIDENCE OF AGE WHICH IS FALSE, FRAUDULENT OR NOT ACTUALLY HIS/HER OWN FOR THE PURPOSE OF ATTEMPTING TO PURCHASE ANY ALCOHOLIC BEVERAGE.( A) Bone marrow stromal cells from 16 week old male Adipoq Cre+/mTmG+ mice were cultured at low density for 14 days to promote formation of colony-forming units (CFUs).

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